BioTek: Microplate Instrumentation & Software Solutions
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ELx405 Select Deep Well Microplate Washer

From the leaders in microplate washing, BioTek introduces the world’s first deep well microplate washer that aspirates and dispenses into 96- and 384-well plates <50 mm in height. ANSI/SBS standard microplates are also accommodated, resulting in a total solution for all of a laboratory’s solid bottom microplate washing needs. The new ELx405™ Select Deep Well Microplate Washer is a robot compatible, full plate washer incorporating BioTek’s patented Dual-Action™ manifold with independent filling and evacuation control for precise overfill washing and overflow protection. Available low-flow rates and angled dispensing make the ELx405 particularly useful in cell-based assays.

Scientists regard unexpected clogging of manifold tubes to be the largest contributor to assay failure. Cleaning these tubes is a time consuming and labor intensive process.  With optional Ultrasonic Advantage™, BioTek washers are the only products available today with built in ultrasonic cleaners able to automatically prime with cleaning solution, sonicate tubes to remove any protein or salt crystal build up and re-prime with rinse or wash buffer leaving the instrument clean and ready for its next wash.

BioTek's Liquid Handling Control Software provides full PC control of the ELx405 Select Deep Well in a familiar Microsoft Windows® environment, while its intuitive StepWise™ protocol creation makes LHC Software easy to use even for novice users.  LHC Secure offers 21 CFR Part 11 compliance with flexible multi-user permission levels and electronic protocol and system audit trail signing additional security is required. 

Applications:

Deep Well Plates
• Plasmid DNA purification
• Serum/plasma sample preparation
• Cell signaling – phospho flow setup for flow cytometry
• Hematopoietic progenitor cell enumeration
• Cell culture – bacterial, yeast and mammalian cells grown in suspension

Standard Height Plates
• ELISAs
• MSD assays
• HCS immune cytochemistry
• FLIPR® Ca2+ flux
• Cell-based assays
• Magnetic bead assays – such as gene expression and cytokine
panels
• ELISPOT assays

 

US Federal Government customers:This product is available on GSA contract. Contact BioTek Customer Care with questions or submit a Quote Request Form to receive pricing and information on specific models (please note 'GSA Contract' in the Comments field).

 

xMAP® is a registered trademark of Luminex® Corporation.
FLIPR® is a registered trademark of Molecular Devices®

Hardware:

  • Washes microplates <50 mm in height
  • Accommodates 96- and 384-well formats
  • Automation of dispense and aspiration steps of conventional ELISA workflows
  • Optional cell washing with gentle, low-flow rates and 20° angled dispense tubes
  • Patented Dual-Action™ manifold for independent control of the aspirate and dispense manifolds for precise overfill washing and overflow protection
  • Internal positive displacement pump eliminates the use of troublesome pressure delivery systems and allows the use of any size dispense reservoir
  • Optional external valve module connects four reagent bottles with automatic switching between reagents
  • Built-in priming trough allows automatic manifold priming and rinsing during both operation and maintenance modes and eliminates the need for blank plates when used in automated robotic systems
  • Optional Ultrasonic Advantage™ – built in ultrasonic cleaner formanifold self-maintenance
  • Built-in fluid flow, fluid detection, and vacuum sensing provide complete protection for unattended operation
  • Biomagnetic separation ready with ANSI/SBS standard height microplates
  • BioStack compatible for unattended processing of up to 50 ANSI/SBS standard height microplates

 



Patented Dual-Action™ manifold design for 96- and 384-well plates.

The ELx405™ Select Deep Well Washer aspirates and dispenses into full size 96- and 384-well blocks with unattended operation.


Software:

  • 75 programs
  • Link up to 10 programs (if valve module is attached, links multiple buffers automatically)
  • Pre/AutoPrime
  • 10 cycles/wash
  • Crosswise aspiration
  • Fully programmable for total control of washing cycles from gentle cell-washing to vigorous wash for high-binding assays
    • Dispense height
    • Aspiration height
    • Aspiration delay
    • Soak
    • Shake
    • Aspiration rate
    • Horizontal aspirate and dispense position
  • External programming/downloading available through USB or RS-232 ports
  • Software control from onboard keypad or Liquid Handling Control™ PC Software
  • 21 CFR Part 11 compliance with LHC™ Secure
Model
Part #
96-/384- well
Deep Well
Standard Height
Cell Washing
Buffer Switching
Ultrasonic
Biomagnetic Separation*
ELx405™ Select Deep Well ELX405UD
ELX405USD
ELX405UVD
ELX405UVSD
ELX405UCWD
ELX405UCWSD
ELX405UCWVD
ELX405UCWVSD

* With available accessory module sold separately

ELx405™ Select Deep Well Microplate Washer Configurations
Feature Design
S Ultrasonic Advantage™
  • Built-in ultrasonic cleaner for manifold self-maintenace
V Automatic 4-Buffer Switching  
U 96-/384-well microplate washing
  • 96-tube (8 x 12) manifold
  • Dual-Action™
D Deep well microplates
  • Accomodates ANSI/SBS standard and deep well microplates <50 mm in height
CW Optimized cell washing for loosely adherent monolayers  

 

Général
Essais ELISA
Cell-based assays (model dependent)
Magnetic bead, polystyrene bead (model dependent)
  • Multiplex assays
  • Bead-based ELISA

Types de microplaque 96- and 384-well
Low profile and standard height
Deep well up to 50 mm
Distribution de liquide Internal positive displacement pump
Agitation Programmable en minutes et en secondes, jusqu'à 60 minutes
Intensités : faible, moyenne, élevée ou variable
Durée de trempage Programmable en minutes et en secondes, jusqu'à 60 minutes
Sécurité/Facilité/Maintenance

Capteur de vide et de déchet, détection de fluide et d’écoulement
Couvercle aérosol
Dispositif d’ajustement pour le positionnement des plaques
Protection débordement
Routines de maintenance préprogrammées

Automatisation Compatible with BioStack (excluding deep well plates) and 3rd party automation
Logiciel embarqué 2 x 24 character backlit LCD display
25 alphanumeric soft keys
Create, edit or run multiple protocols
Logiciel PC Liquid Handling Control ™, pour la programmation du protocole de lavage et de l'exécution PC (en option)
Température de fonctionnement 15 °C  à 30 °C (59 °F à 86 °F)
Interface USB and 9-pin RS232
Humidité 10 % à 85 %, non condensant
Électrique 100 - 240 Volts AC 50/60 Hz, <8,0 A
Lavage
Type de collecteurs Dual-Action 96-tube (8 x 12) manifold
Vitesse de lavage 96-well:
< 30 seconds: 3 aspirate / dispense cycles, 300 µL/well, no soak, final aspirate
384-well:
< 80 seconds: 3 aspirate / dispense cycles, 100 µL/well, no soak, final aspirate
Gamme de volumes 50 - 3000 µL/well
Selectable in 1 µL increments
Sélection de tampons Changement automatique des tampons jusqu'à quatre tampons de lavage (selon les modèles)
Débits 1 - 11
Inclue des taux de lavage de cellules à bas débit (selon le modèle)
Cycles de lavage 1 à 10
Précision de distribution ≤3% CV: 300 µL/well (deionized water with 0.1% Tween 20 and FD&C #1 blue dye), rate of 300µL/well/second, Corning 96-well flat-bottom plate, absorbance read at 630 nm and 450 nm reference
Volume résiduel Standard 96-well plate
<2 µL/well: 3-cycle wash, 300 µL/well (deionized water with 0.1% Tween 20), Corning 96-well flat-bottom plate, aspiration height optimized prior to testing
Deep 96-well plate
<2 µL/well: 3-cycle wash, 2,000 µL/well (deionized water with 0.1% Tween 20), Corning 96-well storage block - P/N 3960 or 3961
Stérilisation Chemical
Nettoyage aux ultrasons Built-in ultrasonic bath for protein and salt crystal buildup
Programmable in hours and minutes, up to 60 minutes (optional)
Volume du flacon d'alimentation 4 L ou 10 L (en option)
Caractéristiques physiques
Dimensions Depth: 17" (43.2 cm)
Width: 14" (35.6 cm)
Height: 11" (27.9 cm)
Poids 30 livres (13,5 kg)
Réglementation
Réglementation Pour usage diagnostique in vitro. Tous les instruments BioTek microplaque sont homologués CE et ETL

Les spécifications sont susceptibles d'être modifiées.

En option

Dispositif d'empilement de microplaques BioStack

Dispositif d'empilement de microplaques BioStack

BioStack™ Microplate Stacker adds stacking capabilities to BioTek's Synergy™, PowerWave™, 405™, ELx405™ Select Deep Well, EL406™, MultiFlo™ and Precision™ product families.


Logiciel Liquid Handling Control

Logiciel Liquid Handling Control

Software is a powerful yet flexible interface for use with BioTek’s liquid handlers including the 405™ Microplate Washer, the ELx405™ Select Deep Well Washer, the EL406™ Washer Dispenser and the MultiFlo™ Dispenser. New LHC Secure offers compliance to the FDA directive 21 CFR Part 11 or whenever additional security is required.


Standard (inclus)

Divers

7102209 Aerosol Cover cet accessoire a une photo

Manuel de l'utilisateur

MANUAL Operator's manuals can be obtained via the Customer Resource Center

En option

Flacons et tubes

7100531 Cap for Waste Bottle, 4L (Also fits 10L or 20L) cet accessoire a une photo
7100532 Spare Cap for 4L Dispense Bottle cet accessoire a une photo
7100533 Waste Tubing Set cet accessoire a une photo
7100534 Waste Bottle, 4L cet accessoire a une photo
7100535 Spare 4L Dispense Bottle Assembly cet accessoire a une photo
7100538 Dispense Tubing Set –1 Buffer cet accessoire a une photo
7100541 Dispense System Complete Assembly, 4L
7100542 Waste Bottle with Level Sensing, 4L cet accessoire a une photo
7100543 Waste Set with Level Sensing, 4L Bottles cet accessoire a une photo
7100544 Cap for Waste Bottle with Level Sensing, 4L cet accessoire a une photo
7100556 Waste Bottle and Tubing, 20L cet accessoire a une photo
7100557 Waste Bottle and Tubing, 10L cet accessoire a une photo
7100559 Dispense Bottle (10L) cet accessoire a une photo
7100593 Spare Cap for 10L Dispense Bottle cet accessoire a une photo

Systèmes de distribution/gestion des déchets

7100581 Dispense System Complete Assembly, 10L cet accessoire a une photo
7100582 Vacuum Set with Level Sense Assembly, 10L cet accessoire a une photo
7100745 Complete Dispense/Waste System 115/230V, 4L Bottles cet accessoire a une photo
7100746 Complete Waste System 115V/230V, 4L Bottles cet accessoire a une photo
7100751 Complete Dispense/High Flow Waste System 115V/230V, 4L Bottles cet accessoire a une photo
7100752 Complete Dispense/High Flow Waste System 115/230V, 10L Bottles cet accessoire a une photo
7100753 Complete High Flow Waste System 115V/230V, 4L Bottles
7100754 High Flow Vacuum Pump 115/230V cet accessoire a une photo
7103024 Standard Vacuum Pump 115V/230V cet accessoire a une photo

Filtres

49943 Dispense Fluid Inlet Screen
48294 Filter for Waste Tubing cet accessoire a une photo

Divers

01113 Silencing Muffler for Standard Vacuum Pump
4030551 Vacuum Gauge/Regulator
7100025 Stylus Kit for any 405™/ELx405™ Select Deep Well manifold cet accessoire a une photo
7102215 384-well Ring Magnet cet accessoire a une photo
7102216 96-well Ring Magnet cet accessoire a une photo
7103016 96-well Flat Magnet cet accessoire a une photo
7103017 384-well Flat Magnet cet accessoire a une photo

Packages d'homologation des produits

7100567 ELx405 Qualification Package

Plaques de test et réactifs

7773001 BioTek Solution #2 10X Concentrate Blue Test Dye (125 mL)
7773002 BioTek Solution #1 100X Concentrate Wetting Agent (125 mL)

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