ELx405 Select Deep Well Microplate Washer
From the leaders in microplate washing, BioTek introduces the world’s first deep well microplate washer that aspirates and dispenses into 96- and 384-well plates <50 mm in height. ANSI/SBS standard microplates are also accommodated, resulting in a total solution for all of a laboratory’s solid bottom microplate washing needs. The new ELx405™ Select Deep Well Microplate Washer is a robot compatible, full plate washer incorporating BioTek’s patented Dual-Action™ manifold with independent filling and evacuation control for precise overfill washing and overflow protection. Available low-flow rates and angled dispensing make the ELx405 particularly useful in cell-based assays.
Scientists regard unexpected clogging of manifold tubes to be the largest contributor to assay failure. Cleaning these tubes is a time consuming and labor intensive process. With optional Ultrasonic Advantage™, BioTek washers are the only products available today with built in ultrasonic cleaners able to automatically prime with cleaning solution, sonicate tubes to remove any protein or salt crystal build up and re-prime with rinse or wash buffer leaving the instrument clean and ready for its next wash.
BioTek's Liquid Handling Control Software provides full PC control of the ELx405 Select Deep Well in a familiar Microsoft Windows® environment, while its intuitive StepWise™ protocol creation makes LHC Software easy to use even for novice users. LHC Secure offers 21 CFR Part 11 compliance with flexible multi-user permission levels and electronic protocol and system audit trail signing additional security is required.
Applications:
Deep Well Plates
• Plasmid DNA purification
• Serum/plasma sample preparation
• Cell signaling – phospho flow setup for flow cytometry
• Hematopoietic progenitor cell enumeration
• Cell culture – bacterial, yeast and mammalian cells grown in suspension
Standard Height Plates
• ELISAs
• MSD assays
• HCS immune cytochemistry
• FLIPR® Ca2+ flux
• Cell-based assays
• Magnetic bead assays – such as gene expression and cytokine
panels
• ELISPOT assays
US Federal Government customers:This product is available on GSA contract. Contact BioTek Customer Care with questions or submit a Quote Request Form to receive pricing and information on specific models (please note 'GSA Contract' in the Comments field).
xMAP® is a registered trademark of Luminex® Corporation.
FLIPR® is a registered trademark of Molecular Devices®
Hardware:
- Washes microplates <50 mm in height
- Accommodates 96- and 384-well formats
- Automation of dispense and aspiration steps of conventional ELISA workflows
- Optional cell washing with gentle, low-flow rates and 20° angled dispense tubes
- Patented Dual-Action™ manifold for independent control of the aspirate and dispense manifolds for precise overfill washing and overflow protection
- Internal positive displacement pump eliminates the use of troublesome pressure delivery systems and allows the use of any size dispense reservoir
- Optional external valve module connects four reagent bottles with automatic switching between reagents
- Built-in priming trough allows automatic manifold priming and rinsing during both operation and maintenance modes and eliminates the need for blank plates when used in automated robotic systems
- Optional Ultrasonic Advantage™ – built in ultrasonic cleaner formanifold self-maintenance
- Built-in fluid flow, fluid detection, and vacuum sensing provide complete protection for unattended operation
- Biomagnetic separation ready with ANSI/SBS standard height microplates
- BioStack compatible for unattended processing of up to 50 ANSI/SBS standard height microplates
Patented Dual-Action™ manifold design for 96- and 384-well plates.
The ELx405™ Select Deep Well Washer aspirates and dispenses into full size 96- and 384-well blocks with unattended operation.
Software:
- 75 programs
- Link up to 10 programs (if valve module is attached, links multiple buffers automatically)
- Pre/AutoPrime
- 10 cycles/wash
- Crosswise aspiration
- Fully programmable for total control of washing cycles from gentle cell-washing to vigorous wash for high-binding assays
- Dispense height
- Aspiration height
- Aspiration delay
- Soak
- Shake
- Aspiration rate
- Horizontal aspirate and dispense position
- External programming/downloading available through USB or RS-232 ports
- Software control from onboard keypad or Liquid Handling Control™ PC Software
- 21 CFR Part 11 compliance with LHC™ Secure
Model |
Part # |
96-/384- well |
Deep Well |
Standard Height |
Cell Washing |
Buffer Switching |
Ultrasonic |
Biomagnetic Separation* |
| ELx405™ Select Deep Well | ELX405UD | • |
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| ELX405USD | • |
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| ELX405UVD | • |
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| ELX405UVSD | • |
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| ELX405UCWD | • |
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| ELX405UCWSD | • |
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| ELX405UCWVD | • |
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| ELX405UCWVSD | • |
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* With available accessory module sold separately
| ELx405™ Select Deep Well Microplate Washer Configurations |
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| Feature | Design | |
| S | Ultrasonic Advantage™ |
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| V | Automatic 4-Buffer Switching | |
| U | 96-/384-well microplate washing |
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| D | Deep well microplates |
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| CW | Optimized cell washing for loosely adherent monolayers | |
| Général | |
| Essais | ELISA Cell-based assays (model dependent) Magnetic bead, polystyrene bead (model dependent)
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| Types de microplaque | 96- and 384-well Low profile and standard height Deep well up to 50 mm |
| Distribution de liquide | Internal positive displacement pump |
| Agitation | Programmable en minutes et en secondes, jusqu'à 60 minutes Intensités : faible, moyenne, élevée ou variable |
| Durée de trempage | Programmable en minutes et en secondes, jusqu'à 60 minutes |
| Sécurité/Facilité/Maintenance | Capteur de vide et de déchet, détection de fluide et d’écoulement |
| Automatisation | Compatible with BioStack (excluding deep well plates) and 3rd party automation |
| Logiciel embarqué | 2 x 24 character backlit LCD display 25 alphanumeric soft keys Create, edit or run multiple protocols |
| Logiciel PC | Liquid Handling Control ™, pour la programmation du protocole de lavage et de l'exécution PC (en option) |
| Température de fonctionnement | 15 °C à 30 °C (59 °F à 86 °F) |
| Interface | USB and 9-pin RS232 |
| Humidité | 10 % à 85 %, non condensant |
| Électrique | 100 - 240 Volts AC 50/60 Hz, <8,0 A |
| Lavage | |
| Type de collecteurs | Dual-Action 96-tube (8 x 12) manifold |
| Vitesse de lavage | 96-well: < 30 seconds: 3 aspirate / dispense cycles, 300 µL/well, no soak, final aspirate 384-well: < 80 seconds: 3 aspirate / dispense cycles, 100 µL/well, no soak, final aspirate |
| Gamme de volumes | 50 - 3000 µL/well Selectable in 1 µL increments |
| Sélection de tampons | Changement automatique des tampons jusqu'à quatre tampons de lavage (selon les modèles) |
| Débits | 1 - 11 Inclue des taux de lavage de cellules à bas débit (selon le modèle) |
| Cycles de lavage | 1 à 10 |
| Précision de distribution | ≤3% CV: 300 µL/well (deionized water with 0.1% Tween 20 and FD&C #1 blue dye), rate of 300µL/well/second, Corning 96-well flat-bottom plate, absorbance read at 630 nm and 450 nm reference |
| Volume résiduel | Standard 96-well plate <2 µL/well: 3-cycle wash, 300 µL/well (deionized water with 0.1% Tween 20), Corning 96-well flat-bottom plate, aspiration height optimized prior to testing Deep 96-well plate <2 µL/well: 3-cycle wash, 2,000 µL/well (deionized water with 0.1% Tween 20), Corning 96-well storage block - P/N 3960 or 3961 |
| Stérilisation | Chemical |
| Nettoyage aux ultrasons | Built-in ultrasonic bath for protein and salt crystal buildup Programmable in hours and minutes, up to 60 minutes (optional) |
| Volume du flacon d'alimentation | 4 L ou 10 L (en option) |
| Caractéristiques physiques | |
| Dimensions | Depth: 17" (43.2 cm) Width: 14" (35.6 cm) Height: 11" (27.9 cm) |
| Poids | 30 livres (13,5 kg) |
| Réglementation | |
| Réglementation | Pour usage diagnostique in vitro. Tous les instruments BioTek microplaque sont homologués CE et ETL |
Les spécifications sont susceptibles d'être modifiées.
En option

Dispositif d'empilement de microplaques BioStack

Logiciel Liquid Handling Control
Standard (inclus) | ||
Divers | ||
| 7102209 | Aerosol Cover |
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Manuel de l'utilisateur | ||
| MANUAL | Operator's manuals can be obtained via the Customer Resource Center | |






