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ELISA and Related Immunoassays

The Enzyme Linked Immunosorbent Assay (ELISA) is a very common, robust technique for detecting various analytes. It spawned a broad range of immunoassays that utilize either wash or no-wash workflows using colorimetric, fluorometric, luminometric, TRF or TR-FRET, AlphaScreen and AlphaLISA detection technologies. Some of these latter technologies utilize low volume analysis in 1536-well plates, yet ELISA performed in 96-well microplates remains the most commonly used immunoassay technique. The ELISA technique causes formation of specific immune complexes that are easily measured using BioTek’s single or multi-mode microplate readers. The basic “building blocks” of an ELISA reaction are:

ELISA process

 

Application Notes:

Learn more about ELISA and Related Immunoassays – see our Applications and technical documentation in Resources.

 

Related Products:

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