Nous utilisons des cookies pour offrir aux visiteurs de notre site web la meilleure expérience possible. Si vous continuez sans changer les paramètres de votre navigateur, nous supposerons que vous êtes heureux de recevoir tous les cookies sur le site web de BioTek. Pour en savoir plus concernant notre utilisations de nos cookies ou comment faire pour bloquer, veuillez consulter notre politique, visite notre cookie policy.

Nucleic Acid Quantification

The quantification of nucleic acids is common to many applications in basic science and clinical research. These assays use either absorbance or fluorescence to measure the concentration of DNA or RNA in the sample. Regardless of the method employed, many laboratories have adapted single cuvette protocols to a 96- and 384-well microplate-based format. These standardized formats, in conjunction with instrumentation capable of recording measurements from them, allow for the rapid quantification of large numbers of samples.

Nucleic acid quantification encompasses key methods including:

  • Spectrophotometric determination of dsDNA, ssDNA, RNA at A260
  • Fluorometric determination of dsDNA with fluorescent dyes, for example, PicoGreen
  • Determination of purity based on A260/A280 ratios

 

Application Notes:

Many more application notes and other technical documentation about Nucleic Acid Quantification are available in Resources.

Related Products:

 

Get more info

 

link