Cell Metabolism: Seahorse XF Imaging and Normalization

Agilent offers an integrated imaging and normalization system with the Seahorse XF analyzer. The system combines cellular analysis and imaging for an intuitive workflow to streamline your XF data interpretation. This imaging and normalization solution combine Agilent strength in cellular metabolic analysis with the Agilent BioTek capability in cell imaging.

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Example of XF data normalization using in situ nuclear staining and in situ cell counting.

Example of XF data normalization using in situ nuclear staining and in situ cell counting.

Figure 1. SKOV3 cells were plated at 1 x 104, 2 x 104, 3 x 104 cells per well, cultured 24 h, subject to the XF Cell Energy Phenotype Test and followed by image analysis. A) Raw OCR and ECAR change with injection (arrows) of oligomycin + FCCP (1.0 and 0.5 µM final, respectively), including 20 µM Hoechst 33342 (2 µM final). B) Representative images of nuclei fluorescently labeled by Hoechst 33342 (upper panel) and nuclei identified and outlined using the Seahorse XF Imaging and Cell Counting software with the Agilent BioTek Cytation 1 (lower panel). C) OCR and ECAR normalized by in situ nuclear staining cell counts (Mean ± SD, n=4).

 

 

Agilent Seahorse XF Imaging Normalization System
Watch this video to see how this solution improves XF data interpretation.

Normalization of Agilent Seahorse XF Data
See the normalization workflow in 4 easy steps.

 

 

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For Research Use Only. Not for use in diagnostic procedures.

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