T Cell Activation and Proliferation

T cell activation is an essential initial step of a targeted immune response. Direct and indirect activation of T cells in vitro causes increased cell proliferation and formation of cell aggregates. This proliferation and aggregation can be tracked and quantitated using label-free and fluorescence imaging methods and analysis.

 

T Cell Activation and Proliferation Assay Procedure

Assay Setup

Step 1. Assay setup: T cells are added to microplate in media containing activating molecules (e.g. anti-CD3 and IL-2).

 

Automated kinetic fluorescence imaging

Step 2. T cell proliferation: T cell expansion results in increased confluence and aggregation detected by Agilent BioTek Gen5 image analysis tools.

 

Automated-analysis

Step 3. Automated analysis: Quantification of T cell proliferation is reported in real time, revealing differential activation levels.

Increase assay throughput and performance

The Agilent BioTek BioSpa compatible assay performs live cell analysis on up to 8 microplates. The Agilent BioTek BioSpa live cell imaging system enables multiplate kinetic analysis of the T cell activation and proliferation assay.
BioSpa
Figure 1. The Agilent BioTek BioSpa Live Cell Analysis System

Liquid handling automation with the Agilent BioTek MultiFlo FX multimode dispenser or other liquid handler can automate seeding cells into the microplate and also conduct media exchanges.

BioSpa

Figure 2. The Agilent BioTek MultiFlo FX multi-mode dispenser.

 

Resources

 

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