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Automated Tissue Culture Cell Fixation and Staining in MicroplatesTélécharger
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January 21, 2014
Authors: Caleb Foster, Paul Held and Peter Banks, BioTek Instruments, Inc.
High Content Screening (HCS) plays a significant role in the drug discovery process, including secondary and toxicity screening. Perhaps the most common HCS application is using antibodies labeled with fluorochromes to localize and quantify proteins within cells. This requires fixing and permeabilizing cells such that the antibodies can penetrate the plasma cell membrane. Often other fluorescent stains are used in addition to identify cells and the nucleus, such as DAPI or Hoechst dyes or cytoskeleton features such as F-actin using fluorescently labeled phalloidin. The workflow for performing these experiments is a laborious multi-step process that also requires multiple cell washing steps within the plate. BioTek's microplate washers have been employed for the washing steps in HCS fixed cell workfl ows for many years and have become an HCS industry standard. In this poster, we detail the use of a combination washer dispenser for automating not only cell washing in microplates, but also all dispense steps including fixative, detergent for permeabilization and fluorescent probes.
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