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Using BioTek’s ELx405™ Select CW Microplate Washer to Wash Loosely Adherent Tissue Culture CellsTélécharger
Related Products: ELx405
December 29, 2004
Author: Paul Held, BioTek Instruments, Winooski, VT 05404
This poster shown at Lab Automation 2005, San Jose, CA, and Miptec 2005, Basel Switzerland
The use of cell cultures in large numbers of samples to test agents has become commonplace in today’s HTS/HCS environments. Screening assays that utilize cells grown in culture often requires one or more wash steps to remove unwanted substances or solutions. For example, prior to microscopic examination of fluorescently stained cells, the media needs to be replaced with an inert buffer such as PBS. After fixation, excess formaldehyde is removed prior to antibody staining, after which the unbound labeled antibody is removed by a washing step. These steps require the addition and removal of buffer solution without disruption of the monolayer of cells present in the wells of the microplate. While the many cultured cells do not present a problem when they are washed, loosely adherent cells often do. Loosely adherent cells do not attach to the microplate substrate strongly and in many instances will form loose aggregates or piles rather than a uniform monolayer. As a result, they are often dislodged when fluid is dispensed at too high a pressure by the plate washer, resulting in their loss with subsequent aspiration of the fluid present in the well. To address these problems with washing loosely adherent cells, BioTek Instruments has developed the ELx405™ Select CW microplate washer. Here we describe the use of the ELx405 Select CW microplate washer to wash the loosely adherent HEK293T cells with PBS in both 96- and 384-well microplates and compare its performance to a standard microplate washer.
Figure 1. ELx405™ Select CW Microplate Washer