DiscoveRx InCELL Hunter Epigenetics Assay reading parameters and fold induction calculations for Z’ and EC50 value

 Related App Note: Automating a Direct, Cell-Based, Target-Compound Interaction Assay for G9a Histone Methyltransferase and Bromodomain Proteins


Gen5 version required: 2.01 or higher



Gen5 software is used to calculate the fold induction from raw luminescence values. The software also calculates a Z’ value from two sets of control wells, as well as a dose response curve from sample wells. An EC50 value for a test compound is finally computed.

Luminescence measurements indicate the level of compound binding to the target epigenetic protein, which can also indicate potential inhibitory characteristics. As shown in this experiment file, positive fold induction is seen with increased binding.