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Differential Cancer Biomarker Secretion of Cultured Human CellsTélécharger
September 30, 2011
Authors: Paul Held, BioTek Instruments, Inc; Kheng Newick, Vermont Cancer Center, University of Vermont, Burlington, Vermont
Diagnostic biomarkers are a key element in cancer research. Research has focused primarily on intracellular biomarkers, such as HER2 or BRAC1, that identify specific tumors, or provide genetic or phenotypic information that can clarify the process of oncogenesis. Recently more attention is being placed on identifying soluble extracellular circulating biomarkers, which can provide information on the body response to cancer, as well as the relationship between a tumor cell and its environment. Because cancer is a series of different disease states, the study of individual biomarkers is usually inadequate to study the complex relationship between a tumor and its environment. Using a panel of known tumor biomarkers to characterize tumor cell lines of known lineage under different conditions provides a better understanding of the biology specific to different tumor types. Using a 22-plex Cancer biomarker assay kit from EMD Millipore we characterized the secretion of cancer biomarkers by several human tissue culture cell lines under normal and low serum conditions. In addition to the established cell lines, three mesothelioma cell lines included two cell lines derived from different individuals were characterized. Comparison data from all cell lines will be provided.