A New Look at Cell-based Assays in a Multi-Mode Microplate Reader using Fluorescence Microscopy

Presenters: Caleb Foster and Paul Held, BioTek Instruments Inc.

Multi-mode microplate readers are a useful tool for cell-based assays. These instruments use PMT-based optics to provide a cell population-averaged assay signal relative to assay controls that can quantify biomolecule expression, activation, inhibition and post-translational modulation. Fluorescence microscopy allows the analysis of sub-populations of cells or even analysis of a single cell, depending on the magnification used.

In this webinar we will demonstrate this dual capability using the Cytation™ 3 Cell imaging Multi-Mode Reader, which incorporates automated digital widefield fluorescence microscopy to perform sub-population analysis of cells in microplates. In addition, the PMT-based optics are used in conjunction with fluorescence microscopy to enable both cell population and sub-population analysis. Examples presented will include quantification of transfection efficiency, target-based inhibition studies with accompanying cell viability determination and phenotypic assays of oxidative stress and hypoxia.