Quantifying Poly-Glutamine Aggregates in C. Elegans Using vivoChip

C. elegans has emerged as a tool for whole organism based high-throughput screening as they model complex human diseases that can not be easily reproduced in vitro. Here, we use a model of Huntington’s disease which consists of poly-glutamine aggregation (PolyQ35:YFP). C. elegans were loaded onto the vivoChip™ (Newormics) and imaged in the YFP channel. Outlines of the worms were identified using Gen5™, and the secondary mask function was used to count the aggregates per worm.